Tfiih Generates a Six-Base-Pair Open Complex during Eukaryotic Transcription Initiation
نویسندگان
چکیده
منابع مشابه
Eukaryotic transcription initiation
Transcription is defined as the synthesis of RNA from a DNA template. In bacteria, transcription of all genes is catalysed by a single RNA polymerase, whereas in the eukaryotic nucleus, three different RNA polymerases — Pol I, Pol II and Pol III — carry out the synthesis of different classes of RNA. Pol I and Pol III transcribe a limited number of genes encoding ribosomal RNAs, transfer RNAs an...
متن کاملEukaryotic Initiation Complex Formation
Two distinct pathways have been elucidated which lead to the formation of an AUG-dependent initiation complex. One pathway involves the use of initiation factor Ml (IF-Ml) to promote AUG-dependent binding of the initiator tRNA to the 40 S subunit, followed by joining of the 60 S subunit in the presence of IF-MBA, IF-MBB, and GTP. The second pathway involves the IF-MP-directed binding of initiat...
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The formation and release of an eukaryotic initiation factor (eIF)-2 X GDP binary complex during eIF-5-mediated assembly of an 80 S ribosomal polypeptide chain initiation complex have been studied by sucrose gradient centrifugation analysis. Isolated 40 S initiation complex reacts with eIF-5 and 60 S ribosomal subunits to form an 80 S ribosomal initiation complex with concomitant hydrolysis of ...
متن کاملRegulation of the initiation of eukaryotic transcription.
DNA sequences that determine transcriptional regulation of a typical eukaryotic gene consist of a core promoter, which serves as a binding site for the GTF TFIID, and regulatory promoter or enhancer sequences, which bind transcriptional activators. The RNA polymerase II transcription machinery consists of over 50 proteins which are thought to bind to the core promoter in as few as two steps: bi...
متن کاملlac Promoter mutation Pr115 generates a new transcription initiation point.
The Eecherichiu coli luc P'115 mutation changes the base-pair where in vitro RNA synthesis initiates from an A/T to a T/A (Maquat et al., 1980). Such an alt,eration results in a catabolite gene activator protein-independent phenotype (Reznikoff, 1976; Maquat et al., 1980). In the absence of catabolite gene activator protein and CAMP, the altered base-pair of the mutant DNA is not used in vitro ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2019
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2018.11.1156